High Glucose Affects Heart Cells in Rats
Author Information
Author(s): Min Wang, Bin Zhang Wen, Quan Zhou Bin, Hui Zhu Jun, Sheng Fu Guo
Primary Institution: The Department of Cardiovascular Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University
Hypothesis
High glucose influences the structure, function, and signaling pathways in cardiomyocytes.
Conclusion
High glucose significantly increased the pulsatile frequency and cellular volumes of cultured cardiomyocytes via the PKC/NF-κB/c-fos pathway, which might lead to diabetic cardiomyopathy.
Supporting Evidence
- Cardiomyocytes in high glucose showed increased pulsatile frequency and cellular volumes.
- The addition of PKC and NF-κB inhibitors partially reversed changes induced by high glucose.
- High glucose levels led to increased expression of PKC-α, PKC-β2, and other related proteins.
Takeaway
When heart cells from baby rats are exposed to high sugar levels, they beat faster and grow bigger, which could lead to heart problems.
Methodology
Cultured cardiomyocytes from neonatal Sprague-Dawley rats were exposed to varying glucose levels, and their pulsatile frequency and cellular volumes were measured.
Participant Demographics
Neonatal Sprague-Dawley rats
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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