How Sumoylation Affects EGR1 Expression and Stability
Author Information
Author(s): Manente Arcangela Gabriella, Pinton Giulia, Tavian Daniela, Lopez-Rodas Gerardo, Brunelli Elisa, Moro Laura
Primary Institution: Dipartimento di Scienze Chimiche, Alimentari, Farmaceutiche e Farmacologiche, University of Piemonte Orientale “A. Avogadro”, Novara, Italy
Hypothesis
The study aims to assess the influence of sumoylation on EGF induced Egr1 expression and/or stability.
Conclusion
The study demonstrates that SUMO-1 modification enhances EGR1 ubiquitination, which is involved in the modulation of its stability upon EGF mediated induction.
Supporting Evidence
- EGR1 is transiently induced upon EGF treatment in ECV304 cells.
- SURMO-1/Ubc9 over-expression stabilizes EGF induced ERK1/2 phosphorylation.
- SUMO-1 modification enhances EGR1 turnover, increasing ubiquitination and proteasome mediated degradation.
Takeaway
When cells are treated with EGF, a protein called EGR1 is made, and a special tag called SUMO helps control how long EGR1 lasts in the cell.
Methodology
The researchers modulated the expression of proteins involved in the sumoylation process in ECV304 cells by transient transfection and evaluated Egr1 expression in response to EGF treatment at mRNA and protein levels.
Digital Object Identifier (DOI)
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