Effects of LOX-1 K167N Mutation on Receptor Activity
Author Information
Author(s): Biocca Silvia, Falconi Mattia, Filesi Ilaria, Baldini Francesco, Vecchione Lucia, Mango Ruggiero, Romeo Francesco, Federici Giorgio, Desideri Alessandro, Novelli Giuseppe
Primary Institution: Department of Neuroscience, University of Tor Vergata, Rome, Italy
Hypothesis
Does the c.501G>C polymorphism change the binding affinity of LOX-1 receptor altering its function?
Conclusion
The K167N mutation in the LOX-1 receptor reduces its ability to bind oxidized low-density lipoprotein (ox-LDL) and impairs its signaling activity.
Supporting Evidence
- The K167N mutation reduces ox-LDL binding and uptake.
- The mutation impairs ERK 1/2 activation in response to ox-LDL.
- Human macrophages with the K167N mutation show decreased LOX-1 induction upon ox-LDL treatment.
Takeaway
A change in a specific part of a protein can make it less effective at grabbing onto bad cholesterol, which might lead to heart problems.
Methodology
The study involved expressing wild-type and mutated LOX-1 in cell lines and analyzing their binding and signaling activities.
Limitations
The study does not confirm the effects of the mutation in vivo or in a larger population.
Participant Demographics
The study involved human macrophages derived from volunteers with different genotypes.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website