Reactive Oxygen Species and Apoptosis in Cancer Treatment
Author Information
Author(s): Brodská Barbora, Holoubek Aleš
Primary Institution: Institute of Hematology and Blood Transfusion, Prague, Czech Republic
Hypothesis
The study investigates the role of reactive oxygen species (ROS) in apoptosis induced by DNA-damaging agents and histone deacetylase inhibitors.
Conclusion
In cancer cell line CML-T1, ROS production significantly contributed to apoptosis triggering, while in normal lymphocytes, both necrosis and apoptosis occurred.
Supporting Evidence
- ROS production significantly contributed to apoptosis in cancer cells.
- Combined treatment with decitabine and SAHA showed synergistic effects in CML cells.
- Normal lymphocytes exhibited both necrosis and apoptosis upon treatment.
Takeaway
The study shows that certain cancer treatments can cause cells to die by making them produce harmful molecules called reactive oxygen species, but normal cells can also be affected in different ways.
Methodology
The study involved treating leukemia cell line CML-T1 and normal peripheral blood lymphocytes with various drugs and measuring cell viability, ROS production, and apoptosis markers.
Limitations
The study notes that the effects on normal lymphocytes showed large variability and that the combination treatments may also induce cell death in normal cells.
Participant Demographics
The study used healthy donors for normal peripheral blood lymphocytes.
Statistical Information
P-Value
<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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