Barcoded Pyrosequencing Reveals That Consumption of Galactooligosaccharides Results in a Highly Specific Bifidogenic Response in Humans
2011

Galactooligosaccharides and Gut Bacteria

Sample size: 18 publication 10 minutes Evidence: moderate

Author Information

Author(s): Davis Lauren M. G., Martínez Inés, Walter Jens, Goin Caitlin, Hutkins Robert W.

Primary Institution: University of Nebraska

Hypothesis

What is the impact of galactooligosaccharides (GOS) on the fecal microbiota of healthy humans?

Conclusion

Consumption of GOS leads to a significant increase in bifidobacteria in the human gut microbiota.

Supporting Evidence

  • GOS consumption led to five- to ten-fold increases in bifidobacteria in half of the subjects.
  • Significant increases in the family Bifidobacteriaceae were observed at doses of 5.0 g and 10.0 g GOS.
  • Consumption of GOS did not alter bacterial diversity of the fecal samples.

Takeaway

Eating certain fibers called galactooligosaccharides can help good bacteria in your tummy grow, especially a type called bifidobacteria.

Methodology

The study used high throughput multiplex community sequencing of 16S rDNA tags to analyze fecal samples from participants consuming varying doses of GOS.

Potential Biases

Potential bias due to individual differences in gut microbiota composition.

Limitations

The response to GOS varied significantly among individuals, with some showing no response.

Participant Demographics

Eighteen healthy adults participated in the study.

Statistical Information

P-Value

<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025200

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