Characterisation of porin genes from Mycobacterium fortuitum and their impact on growth
2009

Study of Porin Genes in Mycobacterium fortuitum and Their Role in Growth

publication Evidence: moderate

Author Information

Author(s): Sharbati Soroush, Schramm Kira, Rempel Sonja, Wang Hwa, Andrich Ronny, Tykiel Verena, Kunisch Ralph, Lewin Astrid

Primary Institution: Freie Universität Berlin

Hypothesis

The growth rate of Mycobacterium fortuitum is influenced by the expression of porin genes.

Conclusion

Porin expression significantly affects the growth and antibiotic susceptibility of Mycobacterium fortuitum.

Supporting Evidence

  • PorM1 and PorM2 enhance the growth of M. fortuitum strains.
  • Repression of porin expression decreases growth rates.
  • Porin expression varies among different strains of M. fortuitum.
  • Over-expression of porins affects antibiotic diffusion into cells.

Takeaway

This study found that certain genes help Mycobacterium fortuitum grow faster and deal with antibiotics better.

Methodology

Porin genes were cloned and characterized, and their effects on growth rates were analyzed using various strains of Mycobacterium fortuitum.

Limitations

The study primarily focused on a limited number of strains and may not represent all Mycobacterium fortuitum variants.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1186/1471-2180-9-31

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