Adenoviral Attachment to Human Platelets
Author Information
Author(s): Nilly Shimony, Gregory Elkin, Dror Kolodkin-Gal, Lina Krasny, Simcha Urieli-Shoval, Yosef S. Haviv
Primary Institution: Hadassah-Hebrew University Medical Center, Jerusalem, Israel
Hypothesis
Can adenoviral vectors activate human platelets and how do they attach to them?
Conclusion
The study developed a flow cytometry method to measure adenoviral attachment to platelets, revealing that adenoviruses can activate platelets and that their binding is mediated by integrins.
Supporting Evidence
- Adenoviral binding to platelets is enhanced by thrombin and MnCl2.
- Platelets lacking the αIIbβ3 integrin can bind adenoviruses more efficiently than normal platelets.
- Integrins are suggested to mediate adenoviral binding to platelets.
Takeaway
This study shows that a virus can stick to and activate blood cells called platelets, which might help us understand how to use this virus in medicine.
Methodology
A direct flow cytometry assay was developed to detect adenoviral particles attached to human platelets, with blocking assays to inhibit attachment and annexin V flow cytometry to analyze platelet activation.
Limitations
The study primarily focused on in vitro conditions, which may not fully replicate in vivo interactions.
Participant Demographics
The study involved human platelets isolated from healthy donors and a patient with Glanzmann thrombasthenia.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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