AspA Protein in Streptococcus pyogenes and Its Role in Biofilm Formation
Author Information
Author(s): Maddocks Sarah E, Wright Christopher J, Nobbs Angela H, Brittan Jane L, Franklin Linda, Strömberg Nicklas, Kadioglu Aras, Jepson Mark A, Jenkinson Howard F
Primary Institution: University of Bristol
Hypothesis
Does the AspA protein in Streptococcus pyogenes play a role in biofilm formation and binding to salivary agglutinin gp-340?
Conclusion
AspA is essential for biofilm formation in Streptococcus pyogenes and mediates binding to salivary agglutinin gp-340.
Supporting Evidence
- AspA binds to immobilized salivary agglutinin gp-340.
- Deletion of aspA gene from S. pyogenes strains abrogated biofilm formation.
- Trans complementation of aspA restored biofilm formation in mutant strains.
- AspA expressed on Lactococcus lactis conferred biofilm-forming ability.
Takeaway
The AspA protein helps Streptococcus pyogenes stick to surfaces in the mouth and form biofilms, which can lead to infections.
Methodology
The study involved expressing AspA in Lactococcus lactis to assess its binding properties and biofilm formation capabilities.
Limitations
The study primarily focused on two serotype M28 strains and may not be generalizable to all S. pyogenes strains.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website