Determinants of Nucleotide-Binding Selectivity of Malic Enzyme
2011
Understanding Malic Enzyme Cofactor Selectivity
publication
Evidence: moderate
Author Information
Author(s): Hsieh Ju-Yi, Chen Meng-Chun, Hung Hui-Chih
Primary Institution: National Chung Hsing University, Taichung, Taiwan
Hypothesis
The study investigates the determinants of nucleotide-binding selectivity in malic enzyme.
Conclusion
The research shows that specific mutations can alter the cofactor preference of malic enzyme from NADP+ to NAD+.
Supporting Evidence
- Mutations in specific residues significantly altered the enzyme's cofactor specificity.
- The study provides kinetic evidence for the role of Glu314 in nucleotide binding.
- The quadruple mutants displayed a shift in cofactor preference from NADP+ to NAD+.
Takeaway
Scientists changed parts of an enzyme to see how it uses different helpers, and found ways to make it prefer one helper over another.
Methodology
The study involved site-directed mutagenesis and kinetic analysis of malic enzyme variants.
Digital Object Identifier (DOI)
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