Induction of Oocyte Maturation and Ovulation in Zebrafish
Author Information
Author(s): Tokumoto Toshinobu, Yamaguchi Toshiya, Ii Sanae, Tokumoto Mika
Primary Institution: National University Corporation Shizuoka University
Hypothesis
Does diethylstilbestrol (DES) as an environmental endocrine disrupting chemical (EDC) affect fish oocyte maturation?
Conclusion
The study demonstrated that DES and other steroids can induce oocyte maturation in zebrafish, while also showing that DES does not induce ovulation.
Supporting Evidence
- DES induced oocyte maturation in zebrafish within several hours.
- 17,20β-DHP also induced oocyte maturation and ovulation.
- PCP inhibited oocyte maturation induced by 17,20β-DHP.
Takeaway
This study found that certain chemicals can help fish eggs mature, but some chemicals can also stop them from hatching.
Methodology
Zebrafish were treated with various hormones and EDCs in water, and the effects on oocyte maturation and ovulation were assessed.
Potential Biases
Potential bias in the selection of chemicals and their concentrations used in the experiments.
Limitations
The study primarily focused on a limited number of chemicals and their effects on oocyte maturation and ovulation.
Participant Demographics
Gravid female zebrafish were used for the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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