The Haploinsufficient Hematopoietic Microenvironment Is Critical to the Pathological Fracture Repair in Murine Models of Neurofibromatosis Type 1
2011

The Role of Hematopoietic Microenvironment in Fracture Healing in Neurofibromatosis Type 1

Sample size: 9 publication 10 minutes Evidence: high

Author Information

Author(s): Wu Xiaohua, Chen Shi, He Yongzheng, Rhodes Steven D., Mohammad Khalid S., Li Xiaohong, Yang Xianlin, Jiang Li, Nalepa Grzegorz, Snider Paige, Robling Alexander G., Clapp D. Wade, Conway Simon J., Guise Theresa A., Yang Feng-Chun

Primary Institution: Indiana University School of Medicine

Hypothesis

The hematopoietic microenvironment plays a critical role in fracture healing in murine models of Neurofibromatosis Type 1.

Conclusion

The study provides evidence that both the loss of Nf1 in osteoblast precursors and haploinsufficiency in the hematopoietic microenvironment are necessary for the skeletal manifestations of Neurofibromatosis Type 1.

Supporting Evidence

  • The study developed murine models that mimic skeletal dysplasias observed in NF1 patients.
  • Adoptive transfer experiments showed that the Nf1 haploinsufficient marrow microenvironment is critical for fracture healing.
  • PeriCre+;Nf1flox/− mice exhibited reduced bone mineral density and impaired fracture healing compared to controls.

Takeaway

This study shows that a special part of the body called the hematopoietic microenvironment is very important for healing broken bones in mice with a genetic condition called Neurofibromatosis Type 1.

Methodology

The study utilized murine models with genetic modifications to investigate the role of the hematopoietic microenvironment in fracture healing.

Limitations

The study is limited to murine models and may not fully replicate human conditions.

Statistical Information

P-Value

p<0.005

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0024917

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