A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress
2008
New Method to Measure Plant Hormones
Sample size: 6
publication
Evidence: high
Author Information
Author(s): Silvia Forcat, Mark H. Bennett, John W. Mansfield, Murray R. Grant
Primary Institution: Imperial College London
Hypothesis
Can we develop a rapid and robust method for measuring phytohormones in plants under stress?
Conclusion
The study presents a new method for accurately measuring three key plant hormones from a single extract, which is rapid and reproducible.
Supporting Evidence
- The method allows for high throughput analysis of phytohormones.
- It accurately measures hormone levels across a physiological dynamic range.
- The method is applicable to various plant species, not just Arabidopsis.
Takeaway
Scientists created a quick way to measure important plant hormones that help plants respond to stress, using just a small amount of plant tissue.
Methodology
The method uses LC/MS to quantify ABA, JA, and SA from a single crude extract of plant tissue.
Limitations
The method may yield lower hormone levels from freeze-dried tissue compared to fresh tissue.
Digital Object Identifier (DOI)
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