Optimizing Antibacterial Activity of Perilla frutescens Leaf
Author Information
Author(s): Kim Dae-Hyun, Kim Young-Chan, Choi Ung-Kyu
Primary Institution: Ulsan National Institute of Science and Technology
Hypothesis
The study investigates the optimal extraction conditions for maximizing the antibacterial activity of Perilla frutescens var. acuta leaf against Staphylococcus aureus.
Conclusion
The study found that the highest antibacterial activity was achieved at an extraction temperature of 75 °C, extraction time of 24 hours, and 45% ethanol concentration.
Supporting Evidence
- The antibacterial activity of the extract was significantly higher at 75 °C and 24 hours of extraction.
- The population of S. aureus decreased from 7.535 log CFU/mL to 4.865 log CFU/mL.
- Ethyl acetate extract showed the highest phenolic content compared to other extracts.
Takeaway
This study shows how to make a plant extract that can kill bad bacteria better by using the right temperature and time.
Methodology
The study used an evolutionary operation-factorial design technique to optimize extraction conditions for antibacterial activity.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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