The Elg1-RFC Clamp-Loading Complex Performs a Role in Sister Chromatid Cohesion
2009
The Role of Elg1-RFC in Sister Chromatid Cohesion
publication
Evidence: high
Author Information
Author(s): Maradeo Marie E., Skibbens Robert V.
Primary Institution: Lehigh University
Hypothesis
Elg1-RFC participates in sister chromatid cohesion and has distinct functions compared to Ctf18-RFC.
Conclusion
Elg1-RFC plays a significant role in sister chromatid cohesion, distinct from Ctf18-RFC, and its deletion can rescue cohesion defects in certain mutant backgrounds.
Supporting Evidence
- Elg1 deletion rescues ctf7 mutant cell temperature sensitivity.
- Over-expression of ELG1 enhances ctf7 mutant cell growth defects.
- Elg1-RFC does not function redundantly with Ctf18-RFC in cohesion establishment.
Takeaway
Elg1 helps keep sister chromatids together during cell division, and when it's missing, the cells can grow better under certain conditions.
Methodology
The study involved creating various yeast mutant strains and analyzing their growth and cohesion characteristics under different conditions.
Digital Object Identifier (DOI)
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