Sub-typing Mycobacterium avium in Cattle
Author Information
Author(s): Ricchi Matteo, Barbieri Gianluca, Taddei Roberta, Belletti Gian L, Carra Elena, Cammi Giuliana, Garbarino Chiara A, Arrigoni Norma
Primary Institution: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna
Hypothesis
The study aims to explore the effectiveness of combining Mini-and Microsatellite loci for sub-typing Mycobacterium avium subsp. paratuberculosis isolates.
Conclusion
The combination of Mini-and Microsatellite approaches effectively discriminates among Mycobacterium avium Type C isolates.
Supporting Evidence
- The study differentiated 84 isolates into 33 clusters.
- Six loci showed relevant allelic variability.
- The intra-herd analysis revealed multiple genotypes in most herds.
Takeaway
Scientists found a way to tell different types of a germ that makes cows sick by looking at tiny parts of their DNA.
Methodology
The study analyzed 84 Mycobacterium avium isolates using MIRU-VNTR and SSR typing methods.
Potential Biases
Potential bias due to non-representative sampling and links related to cattle trading.
Limitations
The sampling was not representative of the whole country, limiting epidemiological conclusions.
Participant Demographics
Isolates were from different cattle herds primarily located in northern Italy.
Statistical Information
P-Value
0.952
Confidence Interval
0.933 to 0.972
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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