Using RNAi to Fight Foot-and-Mouth Disease Virus in Pigs
Author Information
Author(s): Luo Jihuai, Du Junzheng, Gao Shandian, Zhang Guofeng, Sun Jingjing, Cong Guozheng, Shao Junjun, Lin Tong, Chang Huiyun
Primary Institution: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Hypothesis
Can RNA interference (RNAi) effectively inhibit the expression of the porcine integrin αv subunit, the receptor for foot-and-mouth disease virus (FMDV), and reduce FMDV infection in PK-15 cells?
Conclusion
The study demonstrates that RNAi targeting the integrin αv subunit can significantly reduce FMDV replication in PK-15 cells.
Supporting Evidence
- The iαv-PK-15 cells showed an 89.5% reduction in αv mRNA expression.
- Virus titer was reduced by more than 99% when challenged with FMDV.
- RNAi targeting the integrin αv subunit was shown to effectively inhibit FMDV replication.
Takeaway
Scientists created a special tool to help pigs fight a virus that makes them sick by stopping the virus from attaching to their cells.
Methodology
The researchers constructed an RNAi lentiviral vector to target the porcine integrin αv subunit and evaluated its effectiveness in PK-15 cells using real-time qRT-PCR, immunofluorescence, and ELISA.
Limitations
The study primarily focuses on a specific cell line and may not fully represent in vivo conditions.
Statistical Information
P-Value
<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website