Expression and characterization of UL16 gene from duck enteritis virus
2011
Characterization of the UL16 Gene from Duck Enteritis Virus
publication
Evidence: moderate
Author Information
Author(s): He Qin, Yang Qiao, Cheng Anchun, Wang Mingshu, Xiang Jun, Zhu Dekang, Jia Renyong, Luo Qihui, Chen Zhengli, Zhou Yi, Chen Xiaoyue
Primary Institution: Sichuan Agricultural University
Hypothesis
The study aims to analyze and characterize the UL16 gene product of duck enteritis virus (DEV).
Conclusion
The UL16 gene of DEV was successfully cloned, expressed, and detected in DEV infected duck embryo fibroblast cells for the first time.
Supporting Evidence
- The UL16 gene of DEV was composed of 1089 nucleotides and encoded 362 amino acids.
- Multiple sequence alignment suggested that the UL16 gene was highly conserved in the herpesvirus family.
- The UL16 protein localized mostly around the perinuclear cytoplasmic area and in the cytosol of infected cells.
Takeaway
Scientists studied a gene from a virus that affects ducks and found that it behaves similarly to related genes in other viruses.
Methodology
The UL16 gene was cloned, expressed in E. coli, and analyzed using bioinformatics tools and immunofluorescence assays.
Digital Object Identifier (DOI)
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