Protection of Sinorhizobium against Host Cysteine-Rich Antimicrobial Peptides Is Critical for Symbiosis
2011

How Symbiotic Bacteria Survive Host Defenses

publication 10 minutes Evidence: high

Author Information

Author(s): Haag Andreas F., Baloban Mikhail, Sani Monica, Kerscher Bernhard, Pierre Olivier, Farkas Attila, Longhi Renato, Boncompagni Eric, Hérouart Didier, Dall’Angelo Sergio, Kondorosi Eva, Zanda Matteo, Mergaert Peter, Ferguson Gail P.

Primary Institution: University of Aberdeen

Hypothesis

BacA is critical for the bacterial response towards NCR AMPs.

Conclusion

BacA is essential for protecting Sinorhizobium meliloti against the bactericidal effects of host antimicrobial peptides, enabling successful symbiosis.

Supporting Evidence

  • BacA was critical to reduce the amount of NCR AMP-induced membrane permeabilization and bacterial killing in vitro.
  • Wild-type bacteria persisted in the presence of NCR AMPs, while BacA-deficient mutants died rapidly.
  • BacA is essential for the legume symbiosis by protecting S. meliloti against the bactericidal effects of NCR AMPs.

Takeaway

BacA helps bacteria survive in plants by protecting them from harmful substances made by the plants, allowing them to live together happily.

Methodology

The study involved in vitro experiments with S. meliloti strains and the analysis of their responses to NCR AMPs.

Limitations

The study does not explore the exact mechanism by which BacA protects against NCR AMPs.

Statistical Information

P-Value

p≤0.001

Statistical Significance

p≤0.001

Digital Object Identifier (DOI)

10.1371/journal.pbio.1001169

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