Detection of Avian Adeno Virus and Chicken Anemia Virus in Poultry
Author Information
Author(s): Rehman Latif U, Sultan Bakht, Ali Ijaz, Bhatti Muhammad A, Khan Sana U, Zaman Khaliq U, Jahangiri Anila T, Khan Najib U, Iqbal Aqib, Bakht Jehan, Swati Zahoor A, Rehman Muti U
Primary Institution: Institute of Biotechnology and Genetic Engineering, KPK Agricultural University, Peshawar
Hypothesis
Can a duplex PCR assay effectively detect avian adenovirus and chicken anemia virus in poultry in Pakistan?
Conclusion
The duplex PCR assay developed in this study has 100% specificity and high sensitivity to detect AAV and CAV infections in poultry birds.
Supporting Evidence
- The duplex PCR assay showed 100% specificity for detecting both AAV and CAV.
- 96 out of 100 field samples tested positive for AAV DNA.
- All 300 experimental samples were positive for CAV DNA.
- The mortality due to AAV varied from 3% to 90% in different broiler farms.
- Co-infections with E. coli and Staphylococcus enhanced mortality rates.
Takeaway
This study created a new test to quickly find two viruses that make chickens sick, helping farmers save money and time.
Methodology
The study involved developing a duplex PCR assay and testing it on 400 samples from experimental and field sources.
Limitations
The study may not account for all possible viral genotypes or other pathogens affecting poultry.
Participant Demographics
Samples were collected from various poultry farms across Pakistan, including broilers, breeders, and layers.
Digital Object Identifier (DOI)
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