Generating DNA Libraries Using Microarrays
Author Information
Author(s): Nina Svensen, Juan José Díaz-Mochón, Mark Bradley
Primary Institution: School of Chemistry, University of Edinburgh
Hypothesis
Can DNA microarrays be used to efficiently and economically generate libraries of defined pieces of DNA?
Conclusion
The study demonstrates a novel method for generating large DNA libraries from microarrays while keeping the arrays intact for further use.
Supporting Evidence
- The method allows for the generation of DNA libraries containing thousands of defined sequences.
- PCR error rate was calculated to be 9.7×10−3/site/duplication.
- Microarrays can be reused for multiple rounds of DNA production.
Takeaway
This study shows a way to make lots of specific DNA pieces quickly and cheaply using special chips called microarrays.
Methodology
DNA microarrays were used to amplify immobilized DNA sequences followed by PCR amplification, allowing the generation of custom DNA libraries.
Limitations
The technique may have a higher PCR error rate compared to standard methods.
Statistical Information
P-Value
9.7×10−3/site/duplication
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website