Different Capacity of Monocyte Subsets to Phagocytose Iron-Oxide Nanoparticles
Author Information
Author(s): Marcus Settles, Martin Etzrodt, Katja Kosanke, Matthias Schiemann, Alexander Zimmermann, Reinhard Meier, Rickmer Braren, Armin Huber, Ernst J. Rummeny, Ralph Weissleder, Filip K. Swirski, Moritz Wildgruber
Primary Institution: Institut für Radiologie, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany
Hypothesis
To explore the capacity of human CD14+CD16++ and CD14++CD16- monocytes to phagocyte iron-oxide nanoparticles in vitro.
Conclusion
Human monocyte subsets internalize different magnetic nanoparticle preparations differently, resulting in variable loading capacities, imaging phenotypes and likely biological properties.
Supporting Evidence
- CD14++CD16- monocytes displayed a significantly higher uptake of iron-oxide nanoparticles compared to CD14+CD16++ monocytes.
- The uptake of iron-oxide nanoparticles resulted in significantly lower T1 and T2 relaxation times for CD14++CD16- monocytes.
- Cell surface protein expression changed after labeling, indicating that the labeling procedure affects monocyte phenotype.
Takeaway
Some types of white blood cells called monocytes can eat tiny particles differently, which helps doctors see how diseases work using special imaging.
Methodology
Human monocytes were labeled with four different magnetic nanoparticle preparations and their cellular uptake was assessed using flow cytometry, fluorescence microscopy, atomic absorption spectrometry, and MRI.
Limitations
The study's findings may not be applicable to other types of nanoparticles with different surface coatings.
Participant Demographics
15 healthy volunteers (8 male, 7 female, age 37±4 years).
Statistical Information
P-Value
p=0.0015
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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