SOD2's Role in Protecting Rabbit Corneal Endothelial Cells from Oxidative Stress
Author Information
Author(s): Liu Cailing, Ogando Diego, Bonanno Joseph A.
Primary Institution: School of Optometry, Indiana University
Hypothesis
SOD2 plays a significant anti-oxidant role in corneal endothelial cells.
Conclusion
SOD2 is essential for reducing reactive oxygen species and preventing cell apoptosis in rabbit corneal endothelial cells.
Supporting Evidence
- SOD2 expression was reduced by ~80%-90% in cultured rabbit corneal endothelial cells.
- SOD2 activity was decreased by ~70%-80% following knockdown.
- Total ROS levels significantly increased in SOD2 knockdown cells.
- Mitochondrial membrane potential was reduced by 66% in SOD2 knockdown cells.
- Apoptosis increased by 1.5 fold in SOD2 knockdown samples compared to controls.
Takeaway
SOD2 helps keep eye cells healthy by fighting off harmful substances that can cause damage.
Methodology
SOD2 expression and activity were examined using RT-PCR, western blot analysis, and various assays to measure ROS levels and cell apoptosis.
Limitations
The study primarily focuses on rabbit corneal endothelial cells, which may not fully represent human conditions.
Participant Demographics
Young (8 weeks) New Zealand white rabbits were used for cell isolation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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