No Immune Response by Yeast Ndi1 in Rats
Author Information
Author(s): Marella Mathieu, Seo Byoung Boo, Flotte Terence R., Matsuno-Yagi Akemi, Yagi Takao
Primary Institution: The Scripps Research Institute
Hypothesis
Does the expression of the yeast Ndi1 protein in rats trigger an immune response?
Conclusion
The study found that Ndi1 expression did not trigger any inflammatory or immune response in rats.
Supporting Evidence
- Ndi1 expression in rat muscles showed normal morphology without inflammatory infiltrate.
- In contrast, tissues expressing GFP exhibited signs of immune response.
- Ndi1 was localized to mitochondria, while GFP was in the cytosol.
Takeaway
The yeast protein Ndi1 can be safely used in rats without causing an immune reaction, which is good news for gene therapy.
Methodology
Rats were injected with rAAV carrying the NDI1 gene or GFP, and tissues were analyzed for immune response using H&E staining and immunohistochemistry.
Potential Biases
Potential bias in interpreting the absence of immune response due to species differences.
Limitations
The study was conducted in rats, and results may not directly translate to humans.
Participant Demographics
Sprague-Dawley male rats were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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