Efflux Pump and β-Lactam Resistance in Klebsiella pneumoniae
Author Information
Author(s): Jean-Marie Pages, Jean-Philippe Lavigne, Véronique Leflon-Guibout, Estelle Marcon, Frédéric Bert, Latifa Noussair, Marie-Hélène Nicolas-Chanoine
Primary Institution: UMR-MD-1, Facultés de Médecine et de Pharmacie, Université de la Méditerranée, IFR88, Marseille, France
Hypothesis
Assessing the role of efflux pumps in the β-lactam resistance phenotype of Klebsiella pneumoniae isolates.
Conclusion
The study demonstrates that efflux mechanisms significantly contribute to β-lactam susceptibility in clinical isolates of Klebsiella pneumoniae.
Supporting Evidence
- Efflux pump inhibitors significantly decreased MICs of certain antibiotics.
- Clinical isolates displayed a unique β-lactam resistance phenotype.
- Restoration of antibiotic activity was observed with specific combinations of drugs.
Takeaway
Some bacteria can push out antibiotics, making them less effective. This study shows that a specific pump in Klebsiella pneumoniae helps it resist certain antibiotics.
Methodology
The study tested the minimum inhibitory concentrations (MICs) of various β-lactams alone and with an efflux pump inhibitor on clinical isolates.
Limitations
The study focused on a limited number of clinical isolates from a single hospital.
Participant Demographics
The isolates were collected from patients with various infections, primarily from blood samples.
Digital Object Identifier (DOI)
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