Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
2009

Studying Zymomonas mobilis Growth in Different Conditions

publication Evidence: moderate

Author Information

Author(s): Yang Shihui, Tschaplinski Timothy J, Engle Nancy L, Carroll Sue L, Martin Stanton L, Davison Brian H, Palumbo Anthony V, Rodriguez Miguel Jr, Brown Steven D

Primary Institution: Biosciences Division and BioEnergy Science Center, Oak Ridge National Laboratory

Hypothesis

How does Zymomonas mobilis respond to aerobic versus anaerobic fermentation conditions?

Conclusion

High oxygen concentrations during Z. mobilis fermentations negatively influence fermentation performance.

Supporting Evidence

  • Z. mobilis grows faster and consumes glucose more rapidly under anaerobic conditions.
  • Under aerobic conditions, Z. mobilis produces more byproducts like acetate and lactate.
  • Significant differences in gene expression were observed in stationary phase under different oxygen levels.

Takeaway

Zymomonas mobilis grows better and makes more ethanol without oxygen, but when there's too much oxygen, it makes less ethanol and more other stuff.

Methodology

The study used microarray analysis and various chromatography techniques to analyze the growth and metabolite production of Z. mobilis under different oxygen conditions.

Limitations

The study's findings may not fully represent Z. mobilis behavior in industrial settings due to the controlled conditions used.

Statistical Information

P-Value

0.009

Statistical Significance

p<0.1

Digital Object Identifier (DOI)

10.1186/1471-2164-10-34

Want to read the original?

Access the complete publication on the publisher's website

View Original Publication