Controlling Growth of Neurons and Glia on Parylene-C Patterns
Author Information
Author(s): Delivopoulos Evangelos, Murray Alan F.
Primary Institution: The University of Cambridge
Hypothesis
How does glial division affect neuronal network development on engineered substrates?
Conclusion
The study shows that glial mitosis disrupts neuronal patterns, but this can be mitigated by using Ara-C and BDNF.
Supporting Evidence
- Glial mitosis disrupts neuronal patterns over time.
- The addition of Ara-C preserves neuronal and glial conformity.
- BDNF mitigates neuronal apoptosis associated with Ara-C treatment.
- Stable nitrogen levels indicate protein presence on Parylene-C.
- Statistical analysis shows significant differences in conformity indexes.
Takeaway
This study found that when glial cells grow too much, they can push neurons off their patterns, but using certain drugs can help keep the neurons in place.
Methodology
The study involved harvesting glia and neurons from rat hippocampus and culturing them on Parylene-C patterns while manipulating glial division with Ara-C and BDNF.
Potential Biases
Potential bias in the selection of cell types and the effects of drug treatments on neuronal health.
Limitations
The study does not address long-term effects beyond three weeks or the stability of the Parylene-C patterns over extended periods.
Participant Demographics
Sprague-Dawley rats, postnatal days 1-7.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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