Hydrogen Sulphide Production in Uterine Tissues
Author Information
Author(s): Patel Pushpa, Vatish Manu, Heptinstall John, Wang Rui, Carson Ray J
Primary Institution: College of Medical & Dental Sciences, University of Birmingham
Hypothesis
The study aims to investigate the endogenous production of hydrogen sulphide in rat and human intrauterine tissues in vitro.
Conclusion
Rat and human intrauterine tissues produce hydrogen sulphide in vitro possibly via CBS and CSE enzymes.
Supporting Evidence
- Hydrogen sulphide production rates were highest in rat liver compared to intrauterine tissues.
- Nitric oxide significantly increased hydrogen sulphide production in rat fetal membranes.
- Low oxygen conditions elevated hydrogen sulphide production in human placenta and rat intrauterine tissues.
Takeaway
The study found that both rat and human tissues in the uterus can make a gas called hydrogen sulphide, which might help with pregnancy.
Methodology
The production of hydrogen sulphide was measured in vitro using a standard technique, and the expression of CBS and CSE was investigated via Western blotting.
Limitations
Samples from pre-eclamptic placentas were not available in sufficient numbers for statistical comparison.
Participant Demographics
Tissue samples were collected from 36 pregnant rats and 4 non-pregnant rats, as well as human placentas from 4 elective Caesarean sections.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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