Lymphocytes and the Dap12 Adaptor Are Key Regulators of Osteoclast Activation Associated with Gonadal Failure Bone Mass Negative Regulators
2007

Lymphocytes and Dap12: Key Regulators of Bone Mass

publication 10 minutes Evidence: moderate

Author Information

Author(s): Adrienne Anginot, Romain Dacquin, Marlène Mazzorana, Pierre Jurdic

Primary Institution: Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, France

Hypothesis

Lymphocytes could act as a co-stimulatory signal to stimulate osteoclastogenesis in Dap12 loss of function mice.

Conclusion

Lymphocytes play a similar role to DAP12 in protecting against bone loss after gonadal failure.

Supporting Evidence

  • Lymphocytes stimulate osteoclast differentiation from Dap12-deficient progenitors in vitro.
  • KΔ75 mice lost 45% of their bone mass after ovariectomy, compared to 30% in control mice.
  • Absence of mature lymphocytes increases bone mass in Dap12-deficient mice.
  • Rag1-/- mice lost more than 50% of their bone mass after ovariectomy.

Takeaway

This study shows that certain immune cells help keep bones healthy, especially when hormones that protect bones are low.

Methodology

The study involved in vivo and in vitro experiments using various mouse models to analyze the role of lymphocytes and DAP12 in osteoclastogenesis and bone mass.

Limitations

The study primarily focuses on mouse models, which may not fully replicate human bone physiology.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0000585

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