Differentiation of Two Types of Mobilized Peripheral Blood Stem Cells
Author Information
Author(s): Jin Ping, Wang Ena, Ren Jiaqiang, Childs Richard, Shin Jeong Won, Khuu Hanh, Marincola Francesco M, Stroncek David F
Primary Institution: National Institutes of Health
Hypothesis
miRNA and gene expression analysis would be useful for characterizing HSCs.
Conclusion
HSCs and PBLs have unique miRNA and gene expression profiles.
Supporting Evidence
- miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA.
- Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells.
- Gene expression analysis of the HSCs segregated samples according to mobilization and isolation protocol.
Takeaway
This study looked at different types of blood stem cells and found that they have different tiny molecules that help control their behavior.
Methodology
MicroRNA profiling and gene expression profiling were used to compare different types of mobilized HSCs.
Limitations
Differences in mobilization agents and isolation antibodies may have contributed to the observed differences in stem cell types.
Participant Demographics
Healthy subjects were used for the study.
Statistical Information
P-Value
p<0.005
Statistical Significance
p<0.005
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website