Identifying DDX5 as a New Control for Gene Expression Testing
Author Information
Author(s): Su Li-Jen, Chang Ching-Wei, Wu Yu-Chung, Chen Kuang-Chi, Lin Chien-Ju, Liang Shu-Ching, Lin Chi-Hung, Whang-Peng Jacqueline, Hsu Shih-Lan, Chen Chen-Hsin, Huang Chi-Ying
Primary Institution: Institute of Cancer Research, National Health Research Institutes, Taipei, Taiwan
Hypothesis
Which gene can serve as a better internal control for Q-RT-PCR validation of microarray data?
Conclusion
DDX5 is identified as a more reliable internal control for Q-RT-PCR compared to GAPDH in lung adenocarcinoma samples.
Supporting Evidence
- DDX5 showed a 70% correlation with Q-RT-PCR results compared to 48% for GAPDH.
- The study analyzed 66 microarray slides from lung adenocarcinoma tissues.
- Block bootstrap re-sampling was used to assess gene expression variation.
Takeaway
Researchers found that DDX5 is a better choice than GAPDH for measuring gene expression in lung cancer samples.
Methodology
The study used a block bootstrap re-sampling method to analyze gene expression from 66 Affymetrix microarray slides.
Potential Biases
Potential bias from using only specific internal controls and datasets.
Limitations
The study's findings may not be applicable to all types of tissues or conditions.
Participant Demographics
Patients with lung adenocarcinoma, including paired adjacent normal and tumor samples.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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