Snai1 Deletion Causes Embryonic Death Due to Vascular Issues
Author Information
Author(s): Hilda Lomelí, Christa Starling, Thomas Gridley
Primary Institution: Instituto de Biotecnología, Universidad Nacional Autónoma de México
Hypothesis
What are the effects of epiblast-specific Snai1 deletion on embryonic development?
Conclusion
The study found that the death of Snai1-conditional mutant embryos is due to multiple cardiovascular defects.
Supporting Evidence
- Snai1-cko embryos exhibited defects in left-right asymmetry specification.
- Vascular defects were completely penetrant in Snai1-cko embryos.
- Apoptosis was increased in Snai1-cko allantois explants.
Takeaway
When a specific gene called Snai1 is removed from mouse embryos, it causes serious problems with their blood vessels, leading to their death.
Methodology
The study involved creating mouse embryos with a specific gene deletion and analyzing their vascular development through various experiments, including allantois explant cultures.
Limitations
The study does not clarify whether Snai1 function is required within endothelial cells or in surrounding tissues.
Participant Demographics
Mouse embryos
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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