Enzymatic determination of serum triglycerides with the Gemsaec Fast analyser
1980
Enzymatic Determination of Serum Triglycerides with the Gemsaec Fast Analyser
Sample size: 75000
publication
Evidence: moderate
Author Information
Author(s): P. Bijster, H. L. Vader, C. L. J. Vink
Primary Institution: Clinical Laboratory, St. Joseph Hospital, Eindhoven
Conclusion
The study presents a new enzymatic method for determining serum triglycerides that minimizes interference from hemolysis and bilirubin.
Supporting Evidence
- The method was tested on over 75,000 specimens.
- The study showed good linearity and precision in triglyceride measurements.
- Interference from hemolysis and bilirubin was evaluated and quantified.
Takeaway
This study shows a new way to measure fat levels in blood that works better and faster than older methods.
Methodology
The method involves hydrolyzing triglycerides and measuring absorbance at two wavelengths to determine concentration.
Limitations
The method may still be affected by extreme levels of hemolysis and bilirubin.
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