Sulfatide Binding by CS6 from E. coli
Author Information
Author(s): Lena Jansson, Joshua Tobias, Catharina Jarefjäll, Michael Lebens, Ann-Mari Svennerholm, Susann Teneberg
Primary Institution: University of Gothenburg
Hypothesis
The study investigates the binding of the CS6 adhesin from enterotoxigenic Escherichia coli to sulfatide in the small intestine.
Conclusion
CS6 from E. coli specifically binds to sulfatide, which may contribute to the bacterium's ability to cause infection in susceptible species.
Supporting Evidence
- CS6-expressing E. coli binds specifically to sulfatide.
- The binding of CS6 to sulfatide is inhibited by dextran sulfate.
- Sulfatide is present in the small intestine of humans and rabbits but absent in mice.
Takeaway
This study shows that a part of the E. coli bacteria can stick to a specific fat found in the intestines of some animals, which helps it cause sickness.
Methodology
The binding of CS6 to glycosphingolipids was tested using chromatographic techniques and binding assays with purified proteins.
Limitations
The study primarily focuses on specific binding interactions and does not explore the broader implications of CS6 binding in vivo.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website