Chromogranin A-Derived Peptides Induce Calcium Entry in Human Neutrophils
Author Information
Author(s): Zhang Dan, Shooshtarizadeh Peiman, Laventie Benoît-Joseph, Colin Didier André, Chich Jean-François, Vidic Jasmina, de Barry Jean, Chasserot-Golaz Sylvette, Delalande François, Van Dorsselaer Alain, Schneider Francis, Helle Karen, Aunis Dominique, Prévost Gilles, Metz-Boutigue Marie-Hélène
Primary Institution: INSERM U575, Physiopathologie du Système Nerveux, Strasbourg, France
Hypothesis
Do two antimicrobial peptides derived from chromogranin A induce calcium entry in human neutrophils?
Conclusion
The study demonstrates that the peptides Chromofungin and Catestatin induce calcium entry in neutrophils through a specific pathway involving calmodulin and calcium-independent phospholipase A2.
Supporting Evidence
- The study shows that Chromofungin and Catestatin induce a concentration-dependent increase in intracellular calcium in neutrophils.
- Calcium entry was completely blocked by the SOCs inhibitor 2-APB, indicating the involvement of store-operated calcium channels.
- Proteomic analysis revealed the release of several factors important for inflammation and innate immunity after treatment with the peptides.
Takeaway
Two special proteins from the body can help white blood cells take in calcium, which is important for fighting infections.
Methodology
Human neutrophils were treated with different concentrations of CgA-derived peptides, and calcium mobilization was measured using flow cytometry and calcium imaging.
Participant Demographics
Human neutrophils from healthy donors.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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