Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
2010
Complete Genome Sequence and Characterization of Bacillus Phage TP21-L
publication
Evidence: high
Author Information
Author(s): Klumpp Jochen, Calendar Richard, Loessner Martin J.
Primary Institution: Institute of Food, Nutrition and Health, ETH Zurich
Hypothesis
The study aims to determine the complete genome sequence of a specific TP21 phage isolate.
Conclusion
The TP21-L phage is unique among Bacillus phages and should be re-deposited in collections with a clear nomenclature.
Supporting Evidence
- The TP21-L genome is 37.5 kb in size and encodes 56 open reading frames.
- Seventeen of the open reading frames revealed homologies to proteins of other bacteriophages.
- Ten distinct structural proteins of the virion were identified by peptide mass fingerprinting.
Takeaway
Scientists studied a virus that infects certain bacteria and found that it has a unique genetic makeup, which helps in naming and classifying it correctly.
Methodology
The genome sequence was determined using shotgun cloning and sequencing, followed by manual gap closing.
Limitations
The origins of some phage isolates are unclear, and one isolate may have been lost.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website