The PPARδ Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells
2009
Effects of PPARδ Ligand GW501516 on Mouse Kidney Cells
publication
Evidence: moderate
Author Information
Author(s): Clark Jordan, Nasrallah Rania, Hébert Richard L.
Primary Institution: University of Ottawa
Hypothesis
PPARδ reduces apoptotic responses and stimulates cell growth in the mouse collecting duct.
Conclusion
The PPARδ ligand GW501516 inhibits growth but does not affect apoptosis in mouse inner medullary collecting duct cells.
Supporting Evidence
- GW501516 reduced DNA and protein synthesis by 80%.
- GW501516 did not affect cell viability.
- Anisomycin treatment increased caspase-3 levels, but GW501516 did not alter this effect.
Takeaway
A drug called GW501516 can slow down the growth of certain kidney cells but doesn't make them die when they are stressed.
Methodology
The study involved treating mouse IMCD-K2 cells with GW501516 and measuring DNA and protein synthesis, cell viability, and caspase activity.
Limitations
The study may not fully represent the effects of PPARδ in other cell types or conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website