Identifying T Cell Epitopes in Foot-and-Mouth Disease Virus
Author Information
Author(s): Liu Xin-Sheng, Wang Yong-Lu, Zhang Yong-Guang, Fang Yu-Zhen, Pan Li, Lu Jian-Liang, Zhou Peng, Zhang Zhong-Wang, Jiang Shou-Tian
Primary Institution: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Hypothesis
The study aims to identify H-2d restricted T cell epitopes from the structural protein VP1 of the Foot-and-Mouth Disease Virus.
Conclusion
The study successfully identified two T cell epitopes, pK1 and pD7, which may aid in vaccine development against Foot-and-Mouth Disease Virus.
Supporting Evidence
- The study identified two T cell epitopes, pK1 and pD7, through a combination of bioinformatics and experimental validation.
- Significant lymphocyte proliferation was observed in response to the identified epitopes.
- The identified epitopes showed strong specific reactions with FMDV positive serum.
Takeaway
Researchers found two important pieces of a virus that help the body fight it, which could help make better vaccines for sick animals.
Methodology
The study used bioinformatics to predict T cell epitopes and validated them through in vitro experiments with mouse lymphocytes.
Limitations
The study's findings may not directly apply to natural hosts of FMDV, such as pigs and cattle.
Participant Demographics
Female SPF level six-week-old BALB/c mice were used in the experiments.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website