Mutation of the Mouse Syce1 Gene Disrupts Synapsis and Suggests a Link between Synaptonemal Complex Structural Components and DNA Repair
Author Information
Author(s): Bolcun-Filas Ewelina, Speed Robert, Taggart Mary, Grey Corinne, de Massy Bernard, Benavente Ricardo, Cooke Howard J.
Primary Institution: MRC Human Genetics Unit and Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh, United Kingdom
Hypothesis
Does the mutation of the Syce1 gene affect synapsis and its relationship with DNA repair proteins?
Conclusion
The mutation of the Syce1 gene in mice leads to infertility due to disrupted meiosis and a failure in the formation of the synaptonemal complex.
Supporting Evidence
- Syce1−/− mice are infertile due to meiotic defects.
- Histological analysis showed a lack of follicles in ovaries and postmeiotic cells in testes.
- Meiotic recombination occurs but is not efficiently repaired in the absence of SYCE1.
Takeaway
When a specific gene in mice is broken, they can't make sperm or eggs properly, which means they can't have babies.
Methodology
The Syce1 gene was disrupted in mice using gene targeting, and various analyses were performed to assess the effects on meiosis and synapsis.
Limitations
The study primarily focuses on the effects of the Syce1 mutation without exploring potential compensatory mechanisms or the roles of other genes.
Participant Demographics
The study involved genetically modified mice, specifically focusing on male and female Syce1−/− mice.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website