Role of MAPK in apolipoprotein CIII-induced apoptosis in INS-1E cells
2009
How Apolipoprotein CIII Causes Cell Death in Diabetes
Sample size: 4
publication
Evidence: moderate
Author Information
Author(s): Sol E-ri, Tea Sundsten, Peter Bergsten
Primary Institution: Uppsala University
Hypothesis
ApoCIII-induced β-cell apoptosis is connected to activation of MAPKs.
Conclusion
Elevated levels of apoCIII may contribute to β-cell apoptosis through activation of p38 and ERK1/2.
Supporting Evidence
- ApoCIII increased apoptosis 2-fold in INS-1E cells after 24 hours.
- Phosphorylation of p38 MAPK doubled after 24 hours of apoCIII exposure.
- Inhibition of p38 and ERK1/2 normalized apoptosis levels.
- Calcium influx was linked to ERK1/2 activation by apoCIII.
Takeaway
This study found that a protein called apoCIII can make insulin-producing cells die, which might be bad for people with diabetes.
Methodology
INS-1E cells were treated with apoCIII and apoptosis was measured through DNA fragmentation and MAPK activation was assessed via immunoblotting.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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