Metabolic Profiling of the Response to an Oral Glucose Tolerance Test Detects Subtle Metabolic Changes
Author Information
Author(s): Wopereis Suzan, Rubingh Carina M., van Erk Marjan J., Verheij Elwin R., van Vliet Trinette, Cnubben Nicole H. P., Smilde Age K., van der Greef Jan, van Ommen Ben, Hendriks Henk F. J.
Primary Institution: Department Quality of Life, TNO, Zeist, the Netherlands
Hypothesis
The study aims to demonstrate and quantify the metabolic changes induced by a mild anti-inflammatory drug intervention in overweight individuals.
Conclusion
The study found that subtle metabolic changes were only detectable using metabolic profiling in combination with an oral glucose tolerance test.
Supporting Evidence
- Metabolic profiling revealed that diclofenac treatment resulted in lower plasma levels of uric acid.
- Repeated measurements during the oral glucose tolerance test increased statistical power.
- Multiple metabolic intermediates of the glutathione synthesis pathway showed time-dependent suppression in response to the glucose challenge test.
Takeaway
Researchers looked at how a medicine affected the metabolism of overweight men after they drank a sugary drink, finding small changes that could help understand health better.
Methodology
The study used plasma metabolic profiling and liquid and gas chromatography mass spectrometry to analyze metabolites in overweight men during an oral glucose tolerance test.
Potential Biases
Potential biases may arise from the self-reported medical history and the exclusion of certain individuals based on health criteria.
Limitations
The study was limited to a small sample size of overweight men, which may not be generalizable to other populations.
Participant Demographics
The participants were overweight men with a BMI between 25.1 and 34.0 kg/m2, aged 19 to 60 years.
Statistical Information
P-Value
0.02
Statistical Significance
p=0.02
Digital Object Identifier (DOI)
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