Bacterial artificial chromosomes improve protein production in mammalian cells
Author Information
Author(s): Blaas Leander, Musteanu Monica, Eferl Robert, Bauer Anton, Casanova Emilio
Primary Institution: Ludwig Boltzmann Institute for Cancer Research
Hypothesis
Can Bacterial Artificial Chromosomes (BACs) enhance recombinant protein production in mammalian cells compared to conventional vectors?
Conclusion
Using Bacterial Artificial Chromosomes for protein production significantly increases yield and stability compared to conventional methods.
Supporting Evidence
- BAC-based vectors improved protein yield by a factor of 10 compared to conventional vectors.
- Protein production was stable for at least 30 passages in cell cultures.
- The number of integrated BAC copies correlated with protein production levels.
Takeaway
This study shows that using special DNA pieces called BACs can help make more proteins in cells, and they work better than older methods.
Methodology
HEK 293 cells were transfected with BAC and conventional vectors, and protein production was measured over time.
Limitations
The study primarily focuses on a specific type of protein and cell line, which may limit generalizability.
Digital Object Identifier (DOI)
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