Effects of the deletion of the Escherichia coli frataxin homologue CyaY on the respiratory NADH:ubiquinone oxidoreductase
2007
Role of CyaY in E. coli Complex I Assembly
publication
Evidence: moderate
Author Information
Author(s): Pohl Thomas, Walter Julia, Stolpe Stefan, Soufo Joel H Defeu, Grauman Peter L, Friedrich Thorsten
Primary Institution: University of Freiburg
Hypothesis
Is CyaY a structural component of the E. coli complex I?
Conclusion
CyaY plays a non-essential role in the assembly of complex I in E. coli and is not a structural component.
Supporting Evidence
- Complex I was fully assembled in the cyaY deletion mutant but in reduced amounts.
- CyaY was found in the cytoplasm and not associated with the membrane.
- The amount of complex I was reduced by one third in the cyaY deletion strain.
- Enzymatic activity of complex I was similar in both parental and mutant strains.
Takeaway
CyaY is a protein in E. coli that helps with energy production but isn't a part of the main energy-making machine.
Methodology
The study used a cyaY deletion mutant to investigate the role of CyaY in complex I assembly through various biochemical assays.
Digital Object Identifier (DOI)
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