Using Chromatin Insulators in Lentiviral Vectors
Author Information
Author(s): Troels T. Nielsen, Johan Jakobsson, Nina Rosenqvist, Cecilia Lundberg
Primary Institution: Lund University
Hypothesis
Can incorporating double copies of chromatin insulators into lentiviral vectors improve their safety and performance?
Conclusion
Incorporating double copies of chromatin insulators into lentiviral vectors results in lower viral titers and may limit their use in clinical applications.
Supporting Evidence
- Insulator vectors were produced at significantly lower titers compared to control vectors.
- The reduction in titer was due to a block during the transduction process after reverse transcription.
- Transgene expression was lower in cells transduced with insulator vectors compared to control vectors.
Takeaway
This study found that adding extra pieces to help genes work better in viruses can actually make the viruses less effective at getting into cells.
Methodology
The study involved cloning chromatin insulators into lentiviral vectors and testing their functionality in various cell types.
Limitations
The study did not demonstrate enhanced safety of insulator vectors in clinical settings.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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