Linkage Map of Tomato with Resistance Genes
Author Information
Author(s): Arun Sharma, Liping Zhang, David Niño-Liu, Hamid Ashrafi, Majid R. Foolad
Primary Institution: Texas A&M University
Hypothesis
Can we develop a linkage map for tomato that displays genomic locations of resistance genes?
Conclusion
The study successfully developed a linkage map for tomato that includes various markers and identifies genomic locations of resistance genes.
Supporting Evidence
- The map spanned 1002.4 cM of the tomato genome.
- 115 RFLP, 94 EST, and 41 RGA markers were used in the study.
- Locations of several ESTs coincided with known resistance genes.
- The study identified 91 polymorphic ESTs between the two parent species.
- 41 markers showed significant deviation from expected segregation ratios.
Takeaway
Researchers made a map of tomato genes to help breed better tomatoes that can resist diseases and grow well in tough conditions.
Methodology
The study used an F2 population from a cross between LA2093 and a tomato breeding line, employing 250 DNA markers including RFLPs, ESTs, and RGAs to construct the linkage map.
Limitations
The study may have limitations related to the genetic variability of the wild species and the specific markers used.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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