Efficient Cre/lox Recombination in Zebrafish

publication Evidence: high

Author Information

Author(s): Hans Stefan Kaslin, Jan Freudenreich, Dorian Brand, Michael Brand

Primary Institution: Biotechnology Center and Center for Regenerative Therapies Dresden, Dresden University of Technology, Dresden, Germany

Can the Cre/loxP system be effectively utilized in zebrafish for site-specific recombination?

The study demonstrates that the Cre/lox system can achieve high recombination efficiency in zebrafish using transposon-mediated transgenesis.

Cre-mediated recombination was detectable as early as 4 hours after tamoxifen administration.
Using low doses of tamoxifen allowed for mosaic labeling of single cells.
The study established that transposon-mediated transgenesis significantly improved recombination efficiency compared to previous methods.

This study shows that scientists can use a special tool called Cre/lox to change genes in zebrafish, which helps them understand how genes work.

The researchers used the Tol2 transposon system to create a red-to-green reporter line and tested Cre-mediated recombination with tamoxifen.

The study's findings may not be applicable to all zebrafish lines or other species without further optimization.

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