Expression of COX-1, COX-2, and Prostaglandin E Synthases in Hypertensive Mice
Author Information
Author(s): Zaher A. Radi, Robert Ostroski
Primary Institution: Pfizer Global Research and Development
Hypothesis
This study investigates the cellular localization and distribution of COX-1, COX-2, mPGES-1, and mPGES-2 in organ tissues from a mouse model of human hypertension.
Conclusion
In hypertensive mice, there are significant microanatomic variations in the distribution and cellular localization of COX-1, COX-2, mPGES-1, and mPGES-2, with no differences in expression between genders.
Supporting Evidence
- COX-1 expression was highest in the distal convoluted tubules and collecting ducts of the kidney.
- COX-2 was strongly expressed in the proximal and distal convoluted tubules and alveolar macrophages.
- mPGES-2 was strongly expressed in bronchial and bronchiolar epithelial cells.
- Expression of these molecules was similar between male and female mice.
Takeaway
The study looked at how certain proteins are found in the lungs, kidneys, and hearts of mice with high blood pressure, and found that these proteins are located in different places in the body.
Methodology
The study used immunohistochemistry to analyze the expression of COX-1, COX-2, mPGES-1, and mPGES-2 in lung, kidney, and heart tissues from hypertensive mice.
Participant Demographics
15 male and 15 female double transgenic mice aged 15-20 weeks.
Digital Object Identifier (DOI)
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