Bone Marrow Suppression by c-Kit Blockade Enhances Tumor Growth of Colorectal Metastases
Author Information
Author(s): Rupertus Kathrin, Haberl Gudrun C. Y., Scheuer Claudia, Menger Michael D., Schilling Martin K., Kollmar Otto
Primary Institution: University of Saarland
Hypothesis
Does targeting the migration of hematopoietic cells through c-Kit blockade affect tumor growth and angiogenesis in colorectal metastases?
Conclusion
Bone marrow suppression via anti-c-Kit treatment enhances tumor cell engraftment of colorectal metastases through the SDF-1/CXCR4 pathway.
Supporting Evidence
- Blockade of c-Kit significantly enhanced tumor cell engraftment compared to controls.
- Neutralization of SDF-1 completely antagonized the tumor growth associated with c-Kit blockade.
- C-Kit blockade increased VEGF and CXCR4 expression within the tumors.
- Histological analysis showed significant tumor cell infiltration in anti-c-Kit treated animals.
Takeaway
When we block a certain protein (c-Kit) that helps blood cells move, tumors can grow faster because the blood cells help them grow. But if we block another protein (SDF-1), it stops the tumors from growing so quickly.
Methodology
BALB/c mice were pretreated with an anti-c-Kit antibody followed by implantation of colorectal cancer cells, with some receiving an anti-SDF-1 antibody.
Potential Biases
Potential bias in the interpretation of results due to the use of a single animal model.
Limitations
The study was conducted in a murine model, which may not fully replicate human tumor biology.
Participant Demographics
Female BALB/c mice, body weight 18–20g.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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