Identifying New Targets of the Pax8 Gene in Thyroid Cells
Author Information
Author(s): Di Palma Tina, Conti Anna, de Cristofaro Tiziana, Scala Serena, Nitsch Lucio, Zannini Mariastella
Primary Institution: Institute of Experimental Endocrinology and Oncology ‘G. Salvatore’ (IEOS), National Research Council, Naples, Italy
Hypothesis
The study aims to identify novel downstream targets of the Pax8 transcription factor in thyroid follicular cells.
Conclusion
The research successfully identified new genes regulated by Pax8, which are important for understanding thyroid disease progression and developing new therapies.
Supporting Evidence
- The study identified 2815 genes that were differentially expressed after Pax8 silencing.
- Novel target genes involved in processes like DNA replication and apoptosis were discovered.
- Validation of gene expression changes was confirmed through qRT-PCR for selected genes.
Takeaway
Scientists studied thyroid cells to find new genes controlled by a protein called Pax8, which helps the thyroid work properly.
Methodology
The study used RNA interference to silence Pax8 in FRTL-5 thyroid cells and analyzed gene expression changes using microarray technology.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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