RDX Affects MicroRNA Expression in Mice
Author Information
Author(s): Zhang Baohong, Pan Xiaoping
Primary Institution: East Carolina University
Hypothesis
Exposure to RDX would induce alterations in miRNA expression, contributing to carcinogenesis.
Conclusion
Environmental toxicant exposure alters the expression of a suite of miRNAs.
Supporting Evidence
- RDX exposure induced significant changes in miRNA expression profiles.
- A total of 113 miRNAs showed significantly altered expression patterns after RDX exposure.
- Many aberrantly expressed miRNAs were related to various cancers and neurotoxicity.
- Brain tissues exhibited a greater number of miRNAs with expression alterations compared to liver tissues.
Takeaway
When mice were fed a chemical called RDX, it changed the way certain tiny molecules called miRNAs worked in their brains and livers, which might affect their health.
Methodology
B6C3F1 mice were fed diets with or without 5 mg/kg RDX for 28 days, and miRNA expression was analyzed using microarray and qRT-PCR.
Potential Biases
Potential bias in the selection of miRNAs and the interpretation of their roles in carcinogenesis.
Limitations
The study primarily focuses on a single toxicant and its effects on specific miRNAs without exploring long-term impacts.
Participant Demographics
Female, virgin B6C3F1 mice, aged 9–11 weeks.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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