How Gag Proteins Affect HIV-1 Infectivity
Author Information
Author(s): Matsuoka Saori, Dam Elisabeth, Lecossier Denise, Clavel François, Hance Allan J
Primary Institution: INSERM U941, Paris, France
Hypothesis
Different primary Gag proteins affect HIV-1 replication in various cell types.
Conclusion
Interactions between host-cell factors and Gag proteins significantly influence HIV-1 infectivity, varying by target cell type and Gag sequence origin.
Supporting Evidence
- Viral infectivity was influenced by the nature of the Gag proteins in a target cell-specific manner.
- Treatment with cyclophilin A inhibitors produced biphasic dose-response curves for viral infectivity.
- The extent of viral infectivity impairment by high-dose CypA inhibitors depended on the viral genotype and target cell type.
Takeaway
This study shows that the proteins from HIV-1 can change how well the virus infects different types of cells, and this can be affected by certain treatments.
Methodology
The study compared the infectivity of recombinant HIV-1 viruses expressing Gag-protease sequences from primary isolates in different target cells, assessing the impact of cyclophilin A inhibitors.
Potential Biases
Potential bias in the selection of viral strains and target cells used in the experiments.
Limitations
The study does not address the long-term effects of Gag protein variations on HIV-1 infectivity.
Participant Demographics
Clinical isolates obtained from patients who had never received protease inhibitors.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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